American Journal of Chemical and Biochemical Engineering

Archive

Submit a Manuscript

Publishing with us to make your research visible to the widest possible audience.

Propose a Special Issue

Building a community of authors and readers to discuss the latest research and develop new ideas.

Antibacterial Effects of Crude Methanolic Extract, Ethyl Acetate Extract and Isolated Compounds from the Fruits of Combretum molle (Combretaceae)

Combretum molle species are extensively used in traditional medicine in the Northern regions of Cameroon against inflammation, infections, diabetes, malaria, bleeding, diarrhea, digestive disorders and others as a diuretic as back pain. This study was conducted to evaluate the antibacterial effects of crude methanolic extract, ethyl acetate extract and isolated compounds from the fruits of Combretum molle using the Muller-Hinton solid medium disc method. Isolation of secondary metabolites from the fruits of Combretum molle was done by the means of usual chromatographic techniques and their structures were confirmed on the basis of spectroscopic data and those available in literature. The first phytochemical exploration of the fruits of Combretum molle afforded sitosterol-3-O-β-D-glucoside (1), oleanolic acid (2), mollic acid (3), mollic acid-3-O-β-D-glucoside (4), 3, 5-dihydroxy-4’,7-dimethoxyflavone (5), and 5,4’-dihydroxy-7-methoxyflavone (6). To the best of our knowledge, compounds (5) and (6) are isolated and reported for the first time from this plant. At the concentration of 25mg/ml methanolic extract, ethyl acetate extract and compounds 3, 4 and 5 presented good antibacterial potential on Salmonella typhimurium (ATCC13311) strain with diameters of the inhibition zone of 18.2±0.1mm, 15.3±0.2mm, 14.2± 0.3mm, 20.2±0.1mm and 15.1± 0.2mm respectively. Also compound 4 and 6 were sensitive on Pseudomonas aeruginosa (ATCC27853) with diameters of inhibition of 18.2±0.2 mm and 14.1± 0.4mm respectively. The results were compared to TE30 (tetracyclin), CIP5 (ciprofloxacin), C30 (chloremphinincol) and NA30 (nalidicic acid) used as references. These results are in agreement with the enthnobotanical uses of the plant and some previous reports on pharmacological activities such as anti-inflammatory, antioxidant, anticancer effects of some isolates and their related previously isolated and identified from several plants.

Combretum molle, Fruit, Triterpenes, Flavonoid, Antibacterial Effect

APA Style

Yakai Fawai, Dawe Amadou, Wangso Albert, Fanta Angele, Moussa Djaouda, et al. (2023). Antibacterial Effects of Crude Methanolic Extract, Ethyl Acetate Extract and Isolated Compounds from the Fruits of Combretum molle (Combretaceae). American Journal of Chemical and Biochemical Engineering, 7(1), 7-14. https://doi.org/10.11648/j.ajcbe.20230701.12

ACS Style

Yakai Fawai; Dawe Amadou; Wangso Albert; Fanta Angele; Moussa Djaouda, et al. Antibacterial Effects of Crude Methanolic Extract, Ethyl Acetate Extract and Isolated Compounds from the Fruits of Combretum molle (Combretaceae). Am. J. Chem. Biochem. Eng. 2023, 7(1), 7-14. doi: 10.11648/j.ajcbe.20230701.12

AMA Style

Yakai Fawai, Dawe Amadou, Wangso Albert, Fanta Angele, Moussa Djaouda, et al. Antibacterial Effects of Crude Methanolic Extract, Ethyl Acetate Extract and Isolated Compounds from the Fruits of Combretum molle (Combretaceae). Am J Chem Biochem Eng. 2023;7(1):7-14. doi: 10.11648/j.ajcbe.20230701.12

Copyright © 2023 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

1. De Leo. M, De Tommasi. N, Sanogo. R, D’Angelo. V, Germano. M. P, Bisignano. G, Braca. A, “Triterpenoid saponins from Pteleopsis suberosa stem bark”. Phytochemistry, 2006, 60, 2623-2629.
2. Rademan. S, Lall. N, “Combretum molle, in Underexplored Medicinal Plants from Sub-Saharan Africa: Plants with Therapeutic Potential for Human Health”, Elsevier, 2020; pp. 67-76.
3. Keay. R, W, Y., “Trees of Nigeria. 3rd eds”. Clavevdo Press Oxford, 1989; Pp 146-216.
4. Kerharo. J, Adams. J. G, “Pharmacopée sénégalaise traditionnelle: plantes médicinales et toxiques”, Ed Vigot et Frères, Paris, 1974, 1011.
5. Reham. Z. H, Shaden. E. Al-Motaani, Tarek. Al-Talhi, “Therapeutic and Ameliorative Effects of Active Compounds of Combretum molle in the Treatment and Relief from Wounds in a Diabetes Mellitus Experimental Model, Coatings, 2021; 11 (3), 324.
6. Yéo. D, Koffi. E, Bidié. A. P, Tako. N. A, Bahi. C, Méité. S, Djaman. A. J, Guéde-Guina. F, “In Vitro Anticholinesterase and Inhibitory Effects of the Aqueous Extract of Combretum molle (Combretaceae) Leaf on Rabbit Breathing”, Tropical Journal of Pharmaceutical, 2010; 9 (5) pp 469-473.
7. Fyhrquist. P, Mwasumbi. L, Vuorela. P, Vuorela. H, Hiltunen. R, Murphy. C, “Preliminary antiproliferative effects of some species of Terminalia, Combretum and Pteleopsis collected in Tanzania on some human cancer cell lines”, Fitoterapia, 2006; 77: 358–366.
8. Parusnath. M, Naidoo. Y, Singh. M, Rihan. H, Dewir. Y. H, “Phytochemical Composition of Combretum molle (R. Br. ex G. Don.) Engl. & Diels Leaf and Stem Extracts”, Plants, 2023; 12, 1702.
9. Mirriam. E, Nyenje, Roland. N. N, “Bioactivity of the acetone extract of the stem bark of Combretum molle on selected bacterial pathogens: Preliminary phytochemical screening”, Journal of Medicinal Plants Research, 2012; Vol. 6 (8), pp. 1476-1481.
10. Bolou. G. E. K, Attioua. B, N’guessam. A, C, Coulibaly. A, N’Guessan. J. D, Djaman. A. J, “In vitro evaluation of antibacterial activity of Terminalia glaucescens planch extract on Salmonella typhi and Salmonella typhimurium”, Newsletter of Liege Sciences Royal Society, 2011; vol. 80, P. 772-790.
11. Bauer. A. W, Kirby. W. M. M, Sherris. T. C, Truck. M, “Antibiotical susceptibility testing by a standardized single disc method”. American journal of clinical pathology, 1966; Vol. 45, P. 493-496.
12. Barry. A. L, Thornsberry. C, “Susceptibility test, diffusion test procedure”, American journal of clinical pathology, 1985; Vol. 19, P. 492-500.
13. FSM (French Society of Microbiology), Recommendations of Antibiogram committee of French Society of Microbiology, 2008; P. 49.
14. Adesokan. A. A, Akanji. M. A, “Antibacterial potentials of aqueous extract of Enantia chlorantha stem barck”, African journal of Biotechnology, 2007; P. 2502-2505.
15. Doughari. J. H, Pukuma. M. S, De. N, “Antibacterial effects of blanites aegyptiaca L. Drel and Moringa oleifera Lam. On Salmonella typhi”, African journal of biotechnology, 2007; Vol. 6 (19), P. 2212-2215.
16. Swadesh. S, Amrita. P, Anirban. C, Santanu. P, “A novel compound β-sitosterol-3-O-β-D-glucoside isolated from Azadirachta indica effectively induces apoptosis in leukemic cells by targeting G0/G1 populations”, Indian Journal of Biochemistry & Biophysics, Vol. 57, 2020; pp. 27-32.
17. Mofiz. U. K. N. M, Sagar. H, Md, “Scopoletin and β-sitosterol glucoside from roots of Ipomoea digitata “, Journal of Pharmacognosy and Phytochemistry, 2015; 4 (2): 05-07.
18. Castellano. J. M, Ramos-Romero. S, Perona. J. S, “Oleanolic Acid: Extraction, Characterization and Biological Activity”, Nutrients 2022; 14, 623.
19. Isaac. S. G, Corinne. C, Richard. T. S, Emmanuel. T, Alex. De T. A, Paule. S, Brigitte. V, Sophie. L, Celine. H, Armel. H. N. K, Jean-Marc. M, “A new phenyl alkyl ester and a new combretin triterpene derivative from Combretum fragrans F. Hoffm (Combretaceae) and antiproliferative activity”, Open Chemistry, 2020; 18: 1523–1531.
20. Manish. K. G, Sanjana. S, Adarsh. K. C, Kishore. B, Sosmitha. G, Ajaikumar. B. K, Vikash. K. D, Latha. R, “Antioxidant, anti-tyrosinase and anti-inflammatory activities of 3,5-dihydroxy-4′,7-dimethoxyflavone isolated from the leaves of Alpinia nigra”. Phytomedicine Plus, 2021; 1:100097.
21. Kerkatou. M, Redouane-Salah. A, León. F, Brouard. I, Mosset. P, Menad. A, Ameddah. S, Benayache. S, Bermejo. J, Benayache. F, “Secondary Metabolites and Antioxidant Activity of Limonium duriusculum (de Girard) Kuntze Extracts”, Asian J. Chem, 2016; 28 (12), 2695-2700.
22. CA-FSM/EUCAST (Antibiogram committee of French Society of Microbiology). European Committee on Antimicrobial Susceptibility Testing (CA-SFM/EUCAST), 2016: 117.
23. Dabole. B, Matcheme. M, Moussa. D, Abdou. T, W, Bouba. R, Meli. A, Loura. B, “Antibacterial Effects of Chemical Constituents Isolated from the Roots of Cordia myxa (Boraginaceae) on Salmonella typhi (Enterobacteriaceae)”, American Journal of Chemical and Biochemical Engineering. Vol. 5, No. 1, 2021, pp. 1-7.
24. Xiao-Nian. X, Fang. W, Yi-Ting. Y, Jing. L, Yue-Zhen. L, Xing. Y, “Antibacterial activity and Mode of action of Dihydromyricetin from Ampelopsis”. Molecules, 2019; 24 (15): 2831.