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Antibacterial Effects of Crude Methanolic Extract, Ethyl Acetate Extract and Isolated Compounds from the Fruits of Combretum molle (Combretaceae)

Combretum molle species are extensively used in traditional medicine in the Northern regions of Cameroon against inflammation, infections, diabetes, malaria, bleeding, diarrhea, digestive disorders and others as a diuretic as back pain. This study was conducted to evaluate the antibacterial effects of crude methanolic extract, ethyl acetate extract and isolated compounds from the fruits of Combretum molle using the Muller-Hinton solid medium disc method. Isolation of secondary metabolites from the fruits of Combretum molle was done by the means of usual chromatographic techniques and their structures were confirmed on the basis of spectroscopic data and those available in literature. The first phytochemical exploration of the fruits of Combretum molle afforded sitosterol-3-O-β-D-glucoside (1), oleanolic acid (2), mollic acid (3), mollic acid-3-O-β-D-glucoside (4), 3, 5-dihydroxy-4’,7-dimethoxyflavone (5), and 5,4’-dihydroxy-7-methoxyflavone (6). To the best of our knowledge, compounds (5) and (6) are isolated and reported for the first time from this plant. At the concentration of 25mg/ml methanolic extract, ethyl acetate extract and compounds 3, 4 and 5 presented good antibacterial potential on Salmonella typhimurium (ATCC13311) strain with diameters of the inhibition zone of 18.2±0.1mm, 15.3±0.2mm, 14.2± 0.3mm, 20.2±0.1mm and 15.1± 0.2mm respectively. Also compound 4 and 6 were sensitive on Pseudomonas aeruginosa (ATCC27853) with diameters of inhibition of 18.2±0.2 mm and 14.1± 0.4mm respectively. The results were compared to TE30 (tetracyclin), CIP5 (ciprofloxacin), C30 (chloremphinincol) and NA30 (nalidicic acid) used as references. These results are in agreement with the enthnobotanical uses of the plant and some previous reports on pharmacological activities such as anti-inflammatory, antioxidant, anticancer effects of some isolates and their related previously isolated and identified from several plants.

Combretum molle, Fruit, Triterpenes, Flavonoid, Antibacterial Effect

APA Style

Yakai Fawai, Dawe Amadou, Wangso Albert, Fanta Angele, Moussa Djaouda, et al. (2023). Antibacterial Effects of Crude Methanolic Extract, Ethyl Acetate Extract and Isolated Compounds from the Fruits of Combretum molle (Combretaceae). American Journal of Chemical and Biochemical Engineering, 7(1), 7-14.

ACS Style

Yakai Fawai; Dawe Amadou; Wangso Albert; Fanta Angele; Moussa Djaouda, et al. Antibacterial Effects of Crude Methanolic Extract, Ethyl Acetate Extract and Isolated Compounds from the Fruits of Combretum molle (Combretaceae). Am. J. Chem. Biochem. Eng. 2023, 7(1), 7-14. doi: 10.11648/j.ajcbe.20230701.12

AMA Style

Yakai Fawai, Dawe Amadou, Wangso Albert, Fanta Angele, Moussa Djaouda, et al. Antibacterial Effects of Crude Methanolic Extract, Ethyl Acetate Extract and Isolated Compounds from the Fruits of Combretum molle (Combretaceae). Am J Chem Biochem Eng. 2023;7(1):7-14. doi: 10.11648/j.ajcbe.20230701.12

Copyright © 2023 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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