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Analytical Method Validation for Quantification of Chloramphenicol Residues in Poultry Meal Using a Liquid Chromatography-Tandem Mass Spectrometry

Quantification of Chloramphenicol (CAP) residues in complex matrices such as poultry meal is a tedious analytical procedure. In this study, a rapid and precise liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous detection and quantification of CAP residues in poultry meals. The chromatographic separation of the CAP was performed at 40°C column temperature on a reverse-phase C18 column using a binary gradient pump mode and quantification of CAP was performed by LC-MS/MS in electrospray mode. Mobile phase constituents were solvent (a) deionized water, and (b) acetonitrile. The flow rate was 0.35 mL/min and the entire run time was 5 min. The method was validated according to 2021/808/EC guidelines, and acceptance criteria for specificity, linearity, recovery, and precision were met in all the cases. The relative standard deviation (RSD) for precision was < 11% for all the cases. The linearity of the calibration curves was excellent (R2 > 0.999) at concentrations of 0.25, 0.50, 0.75, 1.0, 2.0, and 5.0 µg/kg for matrix-matched CAP standard, and the range of linearity of this method was 0.0-5.0 μg/kg with R2 value greater than 0.99. The decision limit (CCα) and detection capability (CCβ) were 0.29 µg/kg and 32 µg/kg respectively, and the recovery percentages ranged between 94% and 100 %. The obtained results of the proposed method met the validation criteria and this method could be a precise and highly desirable analytical procedure for rapid and accurate quantification of chloramphenicol residues in poultry meal.

Chloramphenicol Residues, Poultry Meal, LC-MS/MS, Method Development & Validation

Md. Zahangir Hosain, S. M. Shariful Islam, Md. Mostofa Kamal. (2022). Analytical Method Validation for Quantification of Chloramphenicol Residues in Poultry Meal Using a Liquid Chromatography-Tandem Mass Spectrometry. American Journal of Chemical and Biochemical Engineering, 6(2), 44-50.

Copyright © 2022 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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