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Synthesis and Characterisation of Proteins Profile of “Streptomyces coelicolor” A3 (2) Cultured Within an Inert Solid-State matRix and Submerged Fermentation

In this study, an inert solid matrix named 2-Vinylpyridine polymer, was produced, and used as micro-bioreactor, with aims to enhance the production of secondary metabolites, synthetized by a soil dwelling microorganism. coelicolor” A3 (2). To mimic the natural environment of microbial growth, parameters such as pores size (11, 22, 43 μm) of the hydrophilic 2-Vinylpyridine inert matrix and the culture medium were tested in a micro-bioreactor, the influence over the proteins synthetized by “S. coelicolor” A3 (2) were investigated. The produced proteins were characterized using Matrix-Assisted Laser Desorption/Ionization-Time-Of-Flight/Mass Spectrometer (MALDI-TOF/MS). As result, differences in protein profiles of submerged growth and 2-Vinylpyridine Polymeric-matrix growth cultures were observed and bands that showed variability were analyzed by mass spectrometry. In conclusion, three proteins present in these bands were revealed, such as Elongation Factor EF-tu which was predominant in submerged growth, while the hypothetical protein SCO7276 and S-adenosylmethionine synthetase were identified in bands detected in protein profile from 2-Vinylpyridine Polymeric-matrix. The enhanced levels of S-adenosylmethionine synthetase are consistent with the elevated levels of secondary metabolite biosynthesis in Streptomyces species.

2-VynilPyridine (2VP), PolyHIPE Polymer (PHP), Streptomyces coelicolor A3 (2), MALDI-TOF/MS, Protein

Teresa Matoso Manguangua Victor, Miguel Filho, Jose Maria Samuco, Paul Kamps, Steve Bull, et al. (2022). Synthesis and Characterisation of Proteins Profile of “Streptomyces coelicolor” A3 (2) Cultured Within an Inert Solid-State matRix and Submerged Fermentation. American Journal of Chemical and Biochemical Engineering, 6(2), 51-67.

Copyright © 2022 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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