Antibacterial Effects of Crude Methanolic Extract, Ethyl Acetate Extract and Isolated Compounds from the Fruits of Combretum molle (Combretaceae):: Science Publishing Group

Antibacterial Effects of Crude Methanolic Extract, Ethyl Acetate Extract and Isolated Compounds from the Fruits of Combretum molle (Combretaceae)

Yakai Fawai¹, Dawe Amadou¹, Wangso Albert¹, Fanta Angele¹, Moussa Djaouda², Taira Vincent¹, Doudja Chantal¹, Loura Benoit^3,4

¹Department of Chemistry, Higher Teachers Training College, University of Maroua, Maroua, Cameroon

²Department of Biological Sciences, Faculty of Science, University of Maroua, Maroua, Cameroon

³Department of Chemistry, Faculty of Science, University of Maroua, Maroua, Cameroon

⁴Department of Refining and Petrochemistry, Faculty of Mines and Petroleum Industries, University of Maroua, Kaele, Cameroon

American Journal of Chemical and Biochemical Engineering, Volume 7, Issue 1, June 2023

Pages: 7-14

Received: May 14, 2023

Accepted: Jun. 08, 2023

Published: Jun. 21, 2023

DOI: 10.11648/j.ajcbe.20230701.12

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Abstract

Combretum molle species are extensively used in traditional medicine in the Northern regions of Cameroon against inflammation, infections, diabetes, malaria, bleeding, diarrhea, digestive disorders and others as a diuretic as back pain. This study was conducted to evaluate the antibacterial effects of crude methanolic extract, ethyl acetate extract and isolated compounds from the fruits of Combretum molle using the Muller-Hinton solid medium disc method. Isolation of secondary metabolites from the fruits of Combretum molle was done by the means of usual chromatographic techniques and their structures were confirmed on the basis of spectroscopic data and those available in literature. The first phytochemical exploration of the fruits of Combretum molle afforded sitosterol-3-O-β-D-glucoside (1), oleanolic acid (2), mollic acid (3), mollic acid-3-O-β-D-glucoside (4), 3, 5-dihydroxy-4’,7-dimethoxyflavone (5), and 5,4’-dihydroxy-7-methoxyflavone (6). To the best of our knowledge, compounds (5) and (6) are isolated and reported for the first time from this plant. At the concentration of 25mg/ml methanolic extract, ethyl acetate extract and compounds 3, 4 and 5 presented good antibacterial potential on Salmonella typhimurium (ATCC13311) strain with diameters of the inhibition zone of 18.2±0.1mm, 15.3±0.2mm, 14.2± 0.3mm, 20.2±0.1mm and 15.1± 0.2mm respectively. Also compound 4 and 6 were sensitive on Pseudomonas aeruginosa (ATCC27853) with diameters of inhibition of 18.2±0.2 mm and 14.1± 0.4mm respectively. The results were compared to TE30 (tetracyclin), CIP5 (ciprofloxacin), C30 (chloremphinincol) and NA30 (nalidicic acid) used as references. These results are in agreement with the enthnobotanical uses of the plant and some previous reports on pharmacological activities such as anti-inflammatory, antioxidant, anticancer effects of some isolates and their related previously isolated and identified from several plants.

Keywords

Combretum molle, Fruit, Triterpenes, Flavonoid, Antibacterial Effect

To cite this article

Yakai Fawai, Dawe Amadou, Wangso Albert, Fanta Angele, Moussa Djaouda, et al. (2023). Antibacterial Effects of Crude Methanolic Extract, Ethyl Acetate Extract and Isolated Compounds from the Fruits of Combretum molle (Combretaceae). American Journal of Chemical and Biochemical Engineering, 7(1), 7-14. https://doi.org/10.11648/j.ajcbe.20230701.12

Copyright © 2023 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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