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Study on Extract Methodology of Total Flavonoids from Ginger and Hydroxyl Radicals Scavenging Effect

Received: 8 September 2016     Accepted: 7 December 2016     Published: 7 January 2017
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Abstract

Extract methodology of total flavonoids from ginger and hydroxyl radicals scavenging effect, were researched in this paper. Methanol concentration, solid-liquid ratio, extraction temperature and time were determined as four single-factor in the experiment. The central points of Box-Benhnken design were selected according to the experimental results of single-factor experiment, the extract process was further optimized by RSM and BBD. The optimum extract conditions were methanol concentration of 60%, solid-liquid ratio of 3:30 (g:mL), extraction temperature of 60°C and time of 3 h, The maximum experimental extraction ratio was 0.497% by RSM. The experimental extraction ratio matched well with the theoretical value of 0.538% by solving the multiple regression equation. RSM has been proved to be an effective technique for optimization of extraction process and the fitted quadratic model has a predictive effect on target extracts. The scavenging effect of ginger extracts, BHT and L-ascorbic acid on•OH with the same concentration were sorted by L-ascorbic acid > ginger extract > BHT, and all the three antioxidant regents displayed a significant dose-effect relationship.

Published in American Journal of Chemical and Biochemical Engineering (Volume 1, Issue 1)
DOI 10.11648/j.ajcbe.20170101.12
Page(s) 7-16
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Extract Methodology, Total Flavonoids, Ginger, Extraction Ratio, Response Surface Methodology, Hydroxyl Radicals Scavenging Effect

References
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[3] Huang W., Xue A., Niu H., etal. Optimized ultrasonic-assisted extraction of flavonoids from Folium eucommiae and evaluation of antioxidant activity in multi-test systems in vitro [J]. 2009, 114(3): 765-1172.
[4] Li Y. H., Jiang B., Zhang T., etal. Antioxidant and free radical-scavenging activities of chickpea protein hydrolysate [J]. Food Chemistry, 2008, 106(2): 444–450.
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[7] Wang ZC, Jiang YR, Liu XW, et al. Response Surface Methodology Optimization of total flavonids from ginger leaf and study on antioxidation effect[J]. Natural product research and developmen, 2015, 27(9): 1582-1588.
[8] Mo KJ, Cheng C. Huang P. et al. Study on extract technology, purification and structural appraisal of flavonoid from ginger [J]. Food science, 2005, 26(9): 229-233.
[9] Gao SY, Ge ZZ. Optimization of extraction technique of flavonoids from ginger by response surface methodology [J]. China condiment, 2012, 37(12): 32-35.
[10] Mu YD. Response surface metnodology and its application in food industry [J]. Jourmal of Zhengzhou Institute of technololy, 2001, 22(3): 91-94.
[11] Amado I. R., Franco D., Sánchez M., etal. Optimization of antioxidant extraction from Solanum tuberosum potato peel waste by surface response methodology [J]. Food Chemistry. 2014, 165, 290-299.
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[13] Liu W., Yu Y., Yang R., etal. Optimization of Total Flavonoid Compound Extraction from Gynura medica Leaf Using Response Surface Methodology and Chemical Composition Analysis [J]. Int. J. Mol. Sci. 2010, 11, 4750-4763.
[14] Li H D. Enzyme-assisted extraction of total flavonoids from Wisteria and study on radicals scavenging effect [J]. Journal of Henan normal university (Natural Science Edition), 2014, 42(03): 79-84.
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  • APA Style

    Li Huiduan, Yu Jianzhong. (2017). Study on Extract Methodology of Total Flavonoids from Ginger and Hydroxyl Radicals Scavenging Effect. American Journal of Chemical and Biochemical Engineering, 1(1), 7-16. https://doi.org/10.11648/j.ajcbe.20170101.12

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    ACS Style

    Li Huiduan; Yu Jianzhong. Study on Extract Methodology of Total Flavonoids from Ginger and Hydroxyl Radicals Scavenging Effect. Am. J. Chem. Biochem. Eng. 2017, 1(1), 7-16. doi: 10.11648/j.ajcbe.20170101.12

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    AMA Style

    Li Huiduan, Yu Jianzhong. Study on Extract Methodology of Total Flavonoids from Ginger and Hydroxyl Radicals Scavenging Effect. Am J Chem Biochem Eng. 2017;1(1):7-16. doi: 10.11648/j.ajcbe.20170101.12

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  • @article{10.11648/j.ajcbe.20170101.12,
      author = {Li Huiduan and Yu Jianzhong},
      title = {Study on Extract Methodology of Total Flavonoids from Ginger and Hydroxyl Radicals Scavenging Effect},
      journal = {American Journal of Chemical and Biochemical Engineering},
      volume = {1},
      number = {1},
      pages = {7-16},
      doi = {10.11648/j.ajcbe.20170101.12},
      url = {https://doi.org/10.11648/j.ajcbe.20170101.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcbe.20170101.12},
      abstract = {Extract methodology of total flavonoids from ginger and hydroxyl radicals scavenging effect, were researched in this paper. Methanol concentration, solid-liquid ratio, extraction temperature and time were determined as four single-factor in the experiment. The central points of Box-Benhnken design were selected according to the experimental results of single-factor experiment, the extract process was further optimized by RSM and BBD. The optimum extract conditions were methanol concentration of 60%, solid-liquid ratio of 3:30 (g:mL), extraction temperature of 60°C and time of 3 h, The maximum experimental extraction ratio was 0.497% by RSM. The experimental extraction ratio matched well with the theoretical value of 0.538% by solving the multiple regression equation. RSM has been proved to be an effective technique for optimization of extraction process and the fitted quadratic model has a predictive effect on target extracts. The scavenging effect of ginger extracts, BHT and L-ascorbic acid on•OH with the same concentration were sorted by L-ascorbic acid > ginger extract > BHT, and all the three antioxidant regents displayed a significant dose-effect relationship.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Study on Extract Methodology of Total Flavonoids from Ginger and Hydroxyl Radicals Scavenging Effect
    AU  - Li Huiduan
    AU  - Yu Jianzhong
    Y1  - 2017/01/07
    PY  - 2017
    N1  - https://doi.org/10.11648/j.ajcbe.20170101.12
    DO  - 10.11648/j.ajcbe.20170101.12
    T2  - American Journal of Chemical and Biochemical Engineering
    JF  - American Journal of Chemical and Biochemical Engineering
    JO  - American Journal of Chemical and Biochemical Engineering
    SP  - 7
    EP  - 16
    PB  - Science Publishing Group
    SN  - 2639-9989
    UR  - https://doi.org/10.11648/j.ajcbe.20170101.12
    AB  - Extract methodology of total flavonoids from ginger and hydroxyl radicals scavenging effect, were researched in this paper. Methanol concentration, solid-liquid ratio, extraction temperature and time were determined as four single-factor in the experiment. The central points of Box-Benhnken design were selected according to the experimental results of single-factor experiment, the extract process was further optimized by RSM and BBD. The optimum extract conditions were methanol concentration of 60%, solid-liquid ratio of 3:30 (g:mL), extraction temperature of 60°C and time of 3 h, The maximum experimental extraction ratio was 0.497% by RSM. The experimental extraction ratio matched well with the theoretical value of 0.538% by solving the multiple regression equation. RSM has been proved to be an effective technique for optimization of extraction process and the fitted quadratic model has a predictive effect on target extracts. The scavenging effect of ginger extracts, BHT and L-ascorbic acid on•OH with the same concentration were sorted by L-ascorbic acid > ginger extract > BHT, and all the three antioxidant regents displayed a significant dose-effect relationship.
    VL  - 1
    IS  - 1
    ER  - 

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Author Information
  • Department of Chemistry and Life Science, Chuxiong Normal University, Chuxiong, P. R. China

  • Department of Chemistry and Life Science, Chuxiong Normal University, Chuxiong, P. R. China

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