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Antibacterial Effects of Crude Methanolic Extract, Ethyl Acetate Extract and Isolated Compounds from the Fruits of Combretum molle (Combretaceae)

Received: 14 May 2023    Accepted: 8 June 2023    Published: 21 June 2023
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Abstract

Combretum molle species are extensively used in traditional medicine in the Northern regions of Cameroon against inflammation, infections, diabetes, malaria, bleeding, diarrhea, digestive disorders and others as a diuretic as back pain. This study was conducted to evaluate the antibacterial effects of crude methanolic extract, ethyl acetate extract and isolated compounds from the fruits of Combretum molle using the Muller-Hinton solid medium disc method. Isolation of secondary metabolites from the fruits of Combretum molle was done by the means of usual chromatographic techniques and their structures were confirmed on the basis of spectroscopic data and those available in literature. The first phytochemical exploration of the fruits of Combretum molle afforded sitosterol-3-O-β-D-glucoside (1), oleanolic acid (2), mollic acid (3), mollic acid-3-O-β-D-glucoside (4), 3, 5-dihydroxy-4’,7-dimethoxyflavone (5), and 5,4’-dihydroxy-7-methoxyflavone (6). To the best of our knowledge, compounds (5) and (6) are isolated and reported for the first time from this plant. At the concentration of 25mg/ml methanolic extract, ethyl acetate extract and compounds 3, 4 and 5 presented good antibacterial potential on Salmonella typhimurium (ATCC13311) strain with diameters of the inhibition zone of 18.2±0.1mm, 15.3±0.2mm, 14.2± 0.3mm, 20.2±0.1mm and 15.1± 0.2mm respectively. Also compound 4 and 6 were sensitive on Pseudomonas aeruginosa (ATCC27853) with diameters of inhibition of 18.2±0.2 mm and 14.1± 0.4mm respectively. The results were compared to TE30 (tetracyclin), CIP5 (ciprofloxacin), C30 (chloremphinincol) and NA30 (nalidicic acid) used as references. These results are in agreement with the enthnobotanical uses of the plant and some previous reports on pharmacological activities such as anti-inflammatory, antioxidant, anticancer effects of some isolates and their related previously isolated and identified from several plants.

Published in American Journal of Chemical and Biochemical Engineering (Volume 7, Issue 1)
DOI 10.11648/j.ajcbe.20230701.12
Page(s) 7-14
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Combretum molle, Fruit, Triterpenes, Flavonoid, Antibacterial Effect

References
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    Yakai Fawai, Dawe Amadou, Wangso Albert, Fanta Angele, Moussa Djaouda, et al. (2023). Antibacterial Effects of Crude Methanolic Extract, Ethyl Acetate Extract and Isolated Compounds from the Fruits of Combretum molle (Combretaceae). American Journal of Chemical and Biochemical Engineering, 7(1), 7-14. https://doi.org/10.11648/j.ajcbe.20230701.12

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    Yakai Fawai; Dawe Amadou; Wangso Albert; Fanta Angele; Moussa Djaouda, et al. Antibacterial Effects of Crude Methanolic Extract, Ethyl Acetate Extract and Isolated Compounds from the Fruits of Combretum molle (Combretaceae). Am. J. Chem. Biochem. Eng. 2023, 7(1), 7-14. doi: 10.11648/j.ajcbe.20230701.12

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    AMA Style

    Yakai Fawai, Dawe Amadou, Wangso Albert, Fanta Angele, Moussa Djaouda, et al. Antibacterial Effects of Crude Methanolic Extract, Ethyl Acetate Extract and Isolated Compounds from the Fruits of Combretum molle (Combretaceae). Am J Chem Biochem Eng. 2023;7(1):7-14. doi: 10.11648/j.ajcbe.20230701.12

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  • @article{10.11648/j.ajcbe.20230701.12,
      author = {Yakai Fawai and Dawe Amadou and Wangso Albert and Fanta Angele and Moussa Djaouda and Taira Vincent and Doudja Chantal and Loura Benoit},
      title = {Antibacterial Effects of Crude Methanolic Extract, Ethyl Acetate Extract and Isolated Compounds from the Fruits of Combretum molle (Combretaceae)},
      journal = {American Journal of Chemical and Biochemical Engineering},
      volume = {7},
      number = {1},
      pages = {7-14},
      doi = {10.11648/j.ajcbe.20230701.12},
      url = {https://doi.org/10.11648/j.ajcbe.20230701.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcbe.20230701.12},
      abstract = {Combretum molle species are extensively used in traditional medicine in the Northern regions of Cameroon against inflammation, infections, diabetes, malaria, bleeding, diarrhea, digestive disorders and others as a diuretic as back pain. This study was conducted to evaluate the antibacterial effects of crude methanolic extract, ethyl acetate extract and isolated compounds from the fruits of Combretum molle using the Muller-Hinton solid medium disc method. Isolation of secondary metabolites from the fruits of Combretum molle was done by the means of usual chromatographic techniques and their structures were confirmed on the basis of spectroscopic data and those available in literature. The first phytochemical exploration of the fruits of Combretum molle afforded sitosterol-3-O-β-D-glucoside (1), oleanolic acid (2), mollic acid (3), mollic acid-3-O-β-D-glucoside (4), 3, 5-dihydroxy-4’,7-dimethoxyflavone (5), and 5,4’-dihydroxy-7-methoxyflavone (6). To the best of our knowledge, compounds (5) and (6) are isolated and reported for the first time from this plant. At the concentration of 25mg/ml methanolic extract, ethyl acetate extract and compounds 3, 4 and 5 presented good antibacterial potential on Salmonella typhimurium (ATCC13311) strain with diameters of the inhibition zone of 18.2±0.1mm, 15.3±0.2mm, 14.2± 0.3mm, 20.2±0.1mm and 15.1± 0.2mm respectively. Also compound 4 and 6 were sensitive on Pseudomonas aeruginosa (ATCC27853) with diameters of inhibition of 18.2±0.2 mm and 14.1± 0.4mm respectively. The results were compared to TE30 (tetracyclin), CIP5 (ciprofloxacin), C30 (chloremphinincol) and NA30 (nalidicic acid) used as references. These results are in agreement with the enthnobotanical uses of the plant and some previous reports on pharmacological activities such as anti-inflammatory, antioxidant, anticancer effects of some isolates and their related previously isolated and identified from several plants.},
     year = {2023}
    }
    

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  • TY  - JOUR
    T1  - Antibacterial Effects of Crude Methanolic Extract, Ethyl Acetate Extract and Isolated Compounds from the Fruits of Combretum molle (Combretaceae)
    AU  - Yakai Fawai
    AU  - Dawe Amadou
    AU  - Wangso Albert
    AU  - Fanta Angele
    AU  - Moussa Djaouda
    AU  - Taira Vincent
    AU  - Doudja Chantal
    AU  - Loura Benoit
    Y1  - 2023/06/21
    PY  - 2023
    N1  - https://doi.org/10.11648/j.ajcbe.20230701.12
    DO  - 10.11648/j.ajcbe.20230701.12
    T2  - American Journal of Chemical and Biochemical Engineering
    JF  - American Journal of Chemical and Biochemical Engineering
    JO  - American Journal of Chemical and Biochemical Engineering
    SP  - 7
    EP  - 14
    PB  - Science Publishing Group
    SN  - 2639-9989
    UR  - https://doi.org/10.11648/j.ajcbe.20230701.12
    AB  - Combretum molle species are extensively used in traditional medicine in the Northern regions of Cameroon against inflammation, infections, diabetes, malaria, bleeding, diarrhea, digestive disorders and others as a diuretic as back pain. This study was conducted to evaluate the antibacterial effects of crude methanolic extract, ethyl acetate extract and isolated compounds from the fruits of Combretum molle using the Muller-Hinton solid medium disc method. Isolation of secondary metabolites from the fruits of Combretum molle was done by the means of usual chromatographic techniques and their structures were confirmed on the basis of spectroscopic data and those available in literature. The first phytochemical exploration of the fruits of Combretum molle afforded sitosterol-3-O-β-D-glucoside (1), oleanolic acid (2), mollic acid (3), mollic acid-3-O-β-D-glucoside (4), 3, 5-dihydroxy-4’,7-dimethoxyflavone (5), and 5,4’-dihydroxy-7-methoxyflavone (6). To the best of our knowledge, compounds (5) and (6) are isolated and reported for the first time from this plant. At the concentration of 25mg/ml methanolic extract, ethyl acetate extract and compounds 3, 4 and 5 presented good antibacterial potential on Salmonella typhimurium (ATCC13311) strain with diameters of the inhibition zone of 18.2±0.1mm, 15.3±0.2mm, 14.2± 0.3mm, 20.2±0.1mm and 15.1± 0.2mm respectively. Also compound 4 and 6 were sensitive on Pseudomonas aeruginosa (ATCC27853) with diameters of inhibition of 18.2±0.2 mm and 14.1± 0.4mm respectively. The results were compared to TE30 (tetracyclin), CIP5 (ciprofloxacin), C30 (chloremphinincol) and NA30 (nalidicic acid) used as references. These results are in agreement with the enthnobotanical uses of the plant and some previous reports on pharmacological activities such as anti-inflammatory, antioxidant, anticancer effects of some isolates and their related previously isolated and identified from several plants.
    VL  - 7
    IS  - 1
    ER  - 

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Author Information
  • Department of Chemistry, Higher Teachers Training College, University of Maroua, Maroua, Cameroon

  • Department of Chemistry, Higher Teachers Training College, University of Maroua, Maroua, Cameroon

  • Department of Chemistry, Higher Teachers Training College, University of Maroua, Maroua, Cameroon

  • Department of Chemistry, Higher Teachers Training College, University of Maroua, Maroua, Cameroon

  • Department of Biological Sciences, Faculty of Science, University of Maroua, Maroua, Cameroon

  • Department of Chemistry, Higher Teachers Training College, University of Maroua, Maroua, Cameroon

  • Department of Chemistry, Higher Teachers Training College, University of Maroua, Maroua, Cameroon

  • Department of Chemistry, Faculty of Science, University of Maroua, Maroua, Cameroon; Department of Refining and Petrochemistry, Faculty of Mines and Petroleum Industries, University of Maroua, Kaele, Cameroon

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