Mixed fruit peels (Banana (BP) and Papaya (PP) bioethanol was produced using Saccharomyces cerevisiae. The proximate and compositional analysis of BP and PP was obtained about 6.67% moisture, 5.75% ash, 82.75% volatile matter, and 5% fixed carbon and 1.1gram, 38.1%, 15.7% and 45.1% extractives, hemicellulose, lignin, and cellulose respectively from BP and 8.165% moisture, 5.5% ash, 81.25% volatile matter and 6% fixed carbon 2.08 gram, 42%, 8.6% and 47.32% extractives, hemicellulose, lignin, and cellulose respectively from PP. After Pretreat with KOH (5% w/v) optimize hydrolysis process parameters based on central composite design (CCD) to maximize fermentable sugars. The optimized hydrolysis conditions were 50:50 w/v% mixing of BP and PP, 1.75% H2SO4, and pH 5. The reducing sugar content was measured by DNS and results 11.737g/ml from fifty (50) grams of BP and PP. The maximum yield of bioethanol was 22.5% recorded after 72 hours. Fourier Transform Infrared Spectroscopy (FTIR) peaks associated with O-H, C-O, and C-H stretching and vibrations confirmed the presence of bioethanol in the product. The result confirms that the combination of BP and PP boosts bioethanol productivity than single peels.
| Published in | American Journal of Chemical and Biochemical Engineering (Volume 8, Issue 2) |
| DOI | 10.11648/j.ajcbe.20240802.12 |
| Page(s) | 45-57 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Bioethanol, Fruit Peels, Pretreatment, Hydrolysis, Fermentation, Saccharomyces cerevisiae, Optimization
| [1] | Vijayanand, C., Kamaraj, S., Karthikeyan, S., & Sriramajayam, S. (2016). Research Article characterization of indigenous biomass. 8(50), 2124–2127. |
| [2] | Tsegu, G., Birri, D. J., Tigu, F., & Tesfaye, A. (2022). Bioethanol production from biodegradable wastes using native yeast isolates from Ethiopian traditional alcoholic beverages. Biocatalysis and Agricultural Biotechnology, 43, 102401. |
| [3] | Jahid, M., Gupta, A., & Sharma, D. K. (2018). Biotechniques Production of Bioethanol from Fruit Wastes (Banana, Papaya, Pineapple and Mango Peels) Under Milder Conditions. 8(3). |
| [4] | Singh, A., & Bishnoi, N. R. (2013). Comparative study of various pretreatment techniques for ethanol production from water hyacinth. Industrial Crops and Products, 44, 283–289. |
| [5] | Salam, N. (2018). Review of ethanol production from fruit wastes. Procedings of Research World International Conference, June, 1–5. |
| [6] | Pavithra, C. S., Devi, S. S., Suneetha, J. W., & Durga Rani, C. V. (2017). Nutritional properties of papaya peel. The Pharma Innovation Journal NAAS Rating TPI, 6(7), 170173. |
| [7] | Mitiku, A. A., & Hatsa, T. M. (2020). Bioethanol production from decaying fruits peel using Saccharomyces cerevisiae. International Journal of Current Research and Academic Review, 8(5), 50–59. |
| [8] | Maisyarah, A., Shiun, J., Nasir, F., & Hashim, H. (2019). Characteristics of Cellulose, Hemicellulose and Lignin of MD2 Pineapple Biomass. 7(December 2018), 79–84. |
| [9] | Kabenge, I., Omulo, G., Banadda, N., Seay, J., Zziwa, A., & Kiggundu, N. (2018). Characterization of Banana Peels Wastes as Potential Slow Pyrolysis Feedstock. Journal of Sustainable Development, 11(2), 14. |
| [10] | Hu, J., Arantes, V., & Saddler, J. N. (2011). The enhancement of enzymatic hydrolysis of lignocellulosic substrates by the addition of accessory enzymes such as xylanase: Is it an additive or synergistic effect? Biotechnology for Biofuels, 4(October). |
| [11] | Guragain, Y. N., De Coninck, J., Husson, F., Durand, A., & Rakshit, S. K. (2011). Comparison of some new pretreatment methods for second generation bioethanol production from wheat straw and water hyacinth. Bioresource Technology, 102(6), 4416–4424. |
| [12] | Faustine, A. S., & Djamaan, A. (2021). Bioethanol Production from Various Agricultural Waste Substrate using Saccharomyces cerevisiae. 16(1), 7–13. |
| [13] | Debebe, D., Gabriel, T., Brhane, Y., Temesgen, A., Nigatu, M., & Marew, T. (2018). Comparative in Vitro Evaluation of Brands of Clotrimazole Cream Formulations Marketed in Ethiopia. Journal of Drug Delivery and Therapeutics, 8(1), 17–22. |
| [14] | Danmaliki, G. I., Muhammad, A. M., Shamsuddeen, A. A., & Usman, B. J. (2016). Bioethanol Production from Banana Peels Bioethanol Production from Banana Peels. June. |
| [15] | Barrera, E., & Cajero, P. (2014). com Characterization of Lignocellulosic Fruit Waste as an Alternative Feedstock for Bioethanol Production. 9(2), 1873–1885. |
| [16] | Ayeni, A. O., Adeeyo, O. A., Oresegun, O. M., & Oladimeji, T. E. (2015). Compositional analysis of lignocellulosic materials: Evaluation of an economically viable method suitable for woody and non-woody biomass. American Journal of Engineering Research, 44, 2320–2847. |
| [17] | Amanullah, A., & Kapilan, R. (2021). Utilization of bioethanol generated from papaw peel waste for hand sanitizer production. 8(Iii), 101–124. |
| [18] | Abdulla, R., Derman, E., Ravintaran, P. T., & Azmah, S. (2017). Fuel Ethanol Production from Papaya Waste using Immobilized Saccharomyces cerevisiae. 2, 112–123. |
APA Style
Tadesse, A. A. (2024). Production and Optimization of Bioethanol from Mixed Banana and Papaya Peels Using Saccharomyces Cerevisiae. American Journal of Chemical and Biochemical Engineering, 8(2), 45-57. https://doi.org/10.11648/j.ajcbe.20240802.12
ACS Style
Tadesse, A. A. Production and Optimization of Bioethanol from Mixed Banana and Papaya Peels Using Saccharomyces Cerevisiae. Am. J. Chem. Biochem. Eng. 2024, 8(2), 45-57. doi: 10.11648/j.ajcbe.20240802.12
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Download@article{10.11648/j.ajcbe.20240802.12,
author = {Asab Alemneh Tadesse},
title = {Production and Optimization of Bioethanol from Mixed Banana and Papaya Peels Using Saccharomyces Cerevisiae
},
journal = {American Journal of Chemical and Biochemical Engineering},
volume = {8},
number = {2},
pages = {45-57},
doi = {10.11648/j.ajcbe.20240802.12},
url = {https://doi.org/10.11648/j.ajcbe.20240802.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcbe.20240802.12},
abstract = {Mixed fruit peels (Banana (BP) and Papaya (PP) bioethanol was produced using Saccharomyces cerevisiae. The proximate and compositional analysis of BP and PP was obtained about 6.67% moisture, 5.75% ash, 82.75% volatile matter, and 5% fixed carbon and 1.1gram, 38.1%, 15.7% and 45.1% extractives, hemicellulose, lignin, and cellulose respectively from BP and 8.165% moisture, 5.5% ash, 81.25% volatile matter and 6% fixed carbon 2.08 gram, 42%, 8.6% and 47.32% extractives, hemicellulose, lignin, and cellulose respectively from PP. After Pretreat with KOH (5% w/v) optimize hydrolysis process parameters based on central composite design (CCD) to maximize fermentable sugars. The optimized hydrolysis conditions were 50:50 w/v% mixing of BP and PP, 1.75% H2SO4, and pH 5. The reducing sugar content was measured by DNS and results 11.737g/ml from fifty (50) grams of BP and PP. The maximum yield of bioethanol was 22.5% recorded after 72 hours. Fourier Transform Infrared Spectroscopy (FTIR) peaks associated with O-H, C-O, and C-H stretching and vibrations confirmed the presence of bioethanol in the product. The result confirms that the combination of BP and PP boosts bioethanol productivity than single peels.
},
year = {2024}
}
TY - JOUR T1 - Production and Optimization of Bioethanol from Mixed Banana and Papaya Peels Using Saccharomyces Cerevisiae AU - Asab Alemneh Tadesse Y1 - 2024/09/29 PY - 2024 N1 - https://doi.org/10.11648/j.ajcbe.20240802.12 DO - 10.11648/j.ajcbe.20240802.12 T2 - American Journal of Chemical and Biochemical Engineering JF - American Journal of Chemical and Biochemical Engineering JO - American Journal of Chemical and Biochemical Engineering SP - 45 EP - 57 PB - Science Publishing Group SN - 2639-9989 UR - https://doi.org/10.11648/j.ajcbe.20240802.12 AB - Mixed fruit peels (Banana (BP) and Papaya (PP) bioethanol was produced using Saccharomyces cerevisiae. The proximate and compositional analysis of BP and PP was obtained about 6.67% moisture, 5.75% ash, 82.75% volatile matter, and 5% fixed carbon and 1.1gram, 38.1%, 15.7% and 45.1% extractives, hemicellulose, lignin, and cellulose respectively from BP and 8.165% moisture, 5.5% ash, 81.25% volatile matter and 6% fixed carbon 2.08 gram, 42%, 8.6% and 47.32% extractives, hemicellulose, lignin, and cellulose respectively from PP. After Pretreat with KOH (5% w/v) optimize hydrolysis process parameters based on central composite design (CCD) to maximize fermentable sugars. The optimized hydrolysis conditions were 50:50 w/v% mixing of BP and PP, 1.75% H2SO4, and pH 5. The reducing sugar content was measured by DNS and results 11.737g/ml from fifty (50) grams of BP and PP. The maximum yield of bioethanol was 22.5% recorded after 72 hours. Fourier Transform Infrared Spectroscopy (FTIR) peaks associated with O-H, C-O, and C-H stretching and vibrations confirmed the presence of bioethanol in the product. The result confirms that the combination of BP and PP boosts bioethanol productivity than single peels. VL - 8 IS - 2 ER -
Ethiopian Institutes of Agricultural Research, National Agricultural Biotechnology Research Center, Holleta, Ethiopia
