Production and Optimization of Bioethanol from Mixed Banana and Papaya Peels Using <i>Saccharomyces Cerevisiae</i>

Asab Alemneh Tadesse; Temesgen Atnafu Yemata

doi:doi:10.11648/j.ajcbe.20240802.12

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Production and Optimization of Bioethanol from Mixed Banana and Papaya Peels Using Saccharomyces Cerevisiae

Asab Alemneh Tadesse^*

, Temesgen Atnafu Yemata

Published in American Journal of Chemical and Biochemical Engineering (Volume 8, Issue 2)

Received: 30 August 2024 Accepted: 18 September 2024 Published: 29 September 2024

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Abstract

Mixed fruit peels (Banana (BP) and Papaya (PP) bioethanol was produced using Saccharomyces cerevisiae. The proximate and compositional analysis of BP and PP was obtained about 6.67% moisture, 5.75% ash, 82.75% volatile matter, and 5% fixed carbon and 1.1gram, 38.1%, 15.7% and 45.1% extractives, hemicellulose, lignin, and cellulose respectively from BP and 8.165% moisture, 5.5% ash, 81.25% volatile matter and 6% fixed carbon 2.08 gram, 42%, 8.6% and 47.32% extractives, hemicellulose, lignin, and cellulose respectively from PP. After Pretreat with KOH (5% w/v) optimize hydrolysis process parameters based on central composite design (CCD) to maximize fermentable sugars. The optimized hydrolysis conditions were 50:50 w/v% mixing of BP and PP, 1.75% H₂SO₄, and pH 5. The reducing sugar content was measured by DNS and results 11.737g/ml from fifty (50) grams of BP and PP. The maximum yield of bioethanol was 22.5% recorded after 72 hours. Fourier Transform Infrared Spectroscopy (FTIR) peaks associated with O-H, C-O, and C-H stretching and vibrations confirmed the presence of bioethanol in the product. The result confirms that the combination of BP and PP boosts bioethanol productivity than single peels.

Published in	American Journal of Chemical and Biochemical Engineering (Volume 8, Issue 2)
DOI	10.11648/j.ajcbe.20240802.12
Page(s)	45-57
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Bioethanol, Fruit Peels, Pretreatment, Hydrolysis, Fermentation, Saccharomyces cerevisiae, Optimization

References

[1]	Abdulla, R., Derman, E., Ravintaran, P. T., & Azmah, S. (2017). Fuel Ethanol Production from Papaya Waste using Immobilized Saccharomyces cerevisiae. 2, 112–123.
[2]	Amanullah, A., & Kapilan, R. (2021). Utilization of bioethanol generated from papaw peel waste for hand sanitizer production. 8(Iii), 101–124.
[3]	Ayeni, A. O., Adeeyo, O. A., Oresegun, O. M., & Oladimeji, T. E. (2015). Compositional analysis of lignocellulosic materials: Evaluation of an economically viable method suitable for woody and non-woody biomass. American Journal of Engineering Research, 44, 2320–2847.
[4]	Barrera, E., & Cajero, P. (2014). com Characterization of Lignocellulosic Fruit Waste as an Alternative Feedstock for Bioethanol Production. 9(2), 1873–1885.
[5]	Danmaliki, G. I., Muhammad, A. M., Shamsuddeen, A. A., & Usman, B. J. (2016). Bioethanol Production from Banana Peels Bioethanol Production from Banana Peels. June. https://doi.org/10.9790/2402-1006025662
[6]	Debebe, D., Gabriel, T., Brhane, Y., Temesgen, A., Nigatu, M., & Marew, T. (2018). Comparative in Vitro Evaluation of Brands of Clotrimazole Cream Formulations Marketed in Ethiopia. Journal of Drug Delivery and Therapeutics, 8(1), 17–22. https://doi.org/10.22270/jddt.v8i1.1546
[7]	Faustine, A. S., & Djamaan, A. (2021). Bioethanol Production from Various Agricultural Waste Substrate using Saccharomyces cerevisiae. 16(1), 7–13. https://doi.org/10.9790/3008-1601030713
[8]	Guragain, Y. N., De Coninck, J., Husson, F., Durand, A., & Rakshit, S. K. (2011). Comparison of some new pretreatment methods for second generation bioethanol production from wheat straw and water hyacinth. Bioresource Technology, 102(6), 4416–4424. https://doi.org/10.1016/j.biortech.2010.11.125
[9]	Hu, J., Arantes, V., & Saddler, J. N. (2011). The enhancement of enzymatic hydrolysis of lignocellulosic substrates by the addition of accessory enzymes such as xylanase: Is it an additive or synergistic effect? Biotechnology for Biofuels, 4(October). https://doi.org/10.1186/1754-6834-4-36
[10]	Kabenge, I., Omulo, G., Banadda, N., Seay, J., Zziwa, A., & Kiggundu, N. (2018). Characterization of Banana Peels Wastes as Potential Slow Pyrolysis Feedstock. Journal of Sustainable Development, 11(2), 14. https://doi.org/10.5539/jsd.v11n2p14
[11]	Maisyarah, A., Shiun, J., Nasir, F., & Hashim, H. (2019). Characteristics of Cellulose, Hemicellulose and Lignin of MD2 Pineapple Biomass. 7(December 2018), 79–84. https://doi.org/10.3303/CET1972014
[12]	Mitiku, A. A., & Hatsa, T. M. (2020). Bioethanol production from decaying fruits peel using Saccharomyces cerevisiae. International Journal of Current Research and Academic Review, 8(5), 50–59.
[13]	Pavithra, C. S., Devi, S. S., Suneetha, J. W., & Durga Rani, C. V. (2017). Nutritional properties of papaya peel. The Pharma Innovation Journal NAAS Rating TPI, 6(7), 170173. https://www.researchgate.net/profile/Jessie_Suneetha_W/publication/324202436_Nutritional_properties_of_papaya_peel/links/5ac779584585151e80a3a216/Nutritional-properties-of-papaya-peel.pdf%0Awww.thepharmajournal.com
[14]	Salam, N. (2018). Review of ethanol production from fruit wastes. Procedings of Research World International Conference, June, 1–5. https://www.researchgate.net/publication/329963633%0AREVIEW
[15]	Singh, A., & Bishnoi, N. R. (2013). Comparative study of various pretreatment techniques for ethanol production from water hyacinth. Industrial Crops and Products, 44, 283–289. https://doi.org/10.1016/j.indcrop.2012.11.026
[16]	Jahid, M., Gupta, A., & Sharma, D. K. (2018). Biotechniques Production of Bioethanol from Fruit Wastes (Banana, Papaya, Pineapple and Mango Peels) Under Milder Conditions. 8(3). https://doi.org/10.4172/2155-9821.1000327
[17]	Tsegu, G., Birri, D. J., Tigu, F., & Tesfaye, A. (2022). Bioethanol production from biodegradable wastes using native yeast isolates from Ethiopian traditional alcoholic beverages. Biocatalysis and Agricultural Biotechnology, 43, 102401. https://doi.org/10.1016/j.bcab.2022.102401
[18]	Vijayanand, C., Kamaraj, S., Karthikeyan, S., & Sriramajayam, S. (2016). Research Article characterization of indigenous biomass. 8(50), 2124–2127.

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APA Style

Tadesse, A. A., Yemata, T. A. (2024). Production and Optimization of Bioethanol from Mixed Banana and Papaya Peels Using Saccharomyces Cerevisiae. American Journal of Chemical and Biochemical Engineering, 8(2), 45-57. https://doi.org/10.11648/j.ajcbe.20240802.12

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ACS Style

Tadesse, A. A.; Yemata, T. A. Production and Optimization of Bioethanol from Mixed Banana and Papaya Peels Using Saccharomyces Cerevisiae. Am. J. Chem. Biochem. Eng. 2024, 8(2), 45-57. doi: 10.11648/j.ajcbe.20240802.12

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AMA Style

Tadesse AA, Yemata TA. Production and Optimization of Bioethanol from Mixed Banana and Papaya Peels Using Saccharomyces Cerevisiae. Am J Chem Biochem Eng. 2024;8(2):45-57. doi: 10.11648/j.ajcbe.20240802.12

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@article{10.11648/j.ajcbe.20240802.12,
  author = {Asab Alemneh Tadesse and Temesgen Atnafu Yemata},
  title = {Production and Optimization of Bioethanol from Mixed Banana and Papaya Peels Using Saccharomyces Cerevisiae
},
  journal = {American Journal of Chemical and Biochemical Engineering},
  volume = {8},
  number = {2},
  pages = {45-57},
  doi = {10.11648/j.ajcbe.20240802.12},
  url = {https://doi.org/10.11648/j.ajcbe.20240802.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcbe.20240802.12},
  abstract = {Mixed fruit peels (Banana (BP) and Papaya (PP) bioethanol was produced using Saccharomyces cerevisiae. The proximate and compositional analysis of BP and PP was obtained about 6.67% moisture, 5.75% ash, 82.75% volatile matter, and 5% fixed carbon and 1.1gram, 38.1%, 15.7% and 45.1% extractives, hemicellulose, lignin, and cellulose respectively from BP and 8.165% moisture, 5.5% ash, 81.25% volatile matter and 6% fixed carbon 2.08 gram, 42%, 8.6% and 47.32% extractives, hemicellulose, lignin, and cellulose respectively from PP. After Pretreat with KOH (5% w/v) optimize hydrolysis process parameters based on central composite design (CCD) to maximize fermentable sugars. The optimized hydrolysis conditions were 50:50 w/v% mixing of BP and PP, 1.75% H2SO4, and pH 5. The reducing sugar content was measured by DNS and results 11.737g/ml from fifty (50) grams of BP and PP. The maximum yield of bioethanol was 22.5% recorded after 72 hours. Fourier Transform Infrared Spectroscopy (FTIR) peaks associated with O-H, C-O, and C-H stretching and vibrations confirmed the presence of bioethanol in the product. The result confirms that the combination of BP and PP boosts bioethanol productivity than single peels.
},
 year = {2024}
}

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TY  - JOUR
T1  - Production and Optimization of Bioethanol from Mixed Banana and Papaya Peels Using Saccharomyces Cerevisiae

AU  - Asab Alemneh Tadesse
AU  - Temesgen Atnafu Yemata
Y1  - 2024/09/29
PY  - 2024
N1  - https://doi.org/10.11648/j.ajcbe.20240802.12
DO  - 10.11648/j.ajcbe.20240802.12
T2  - American Journal of Chemical and Biochemical Engineering
JF  - American Journal of Chemical and Biochemical Engineering
JO  - American Journal of Chemical and Biochemical Engineering
SP  - 45
EP  - 57
PB  - Science Publishing Group
SN  - 2639-9989
UR  - https://doi.org/10.11648/j.ajcbe.20240802.12
AB  - Mixed fruit peels (Banana (BP) and Papaya (PP) bioethanol was produced using Saccharomyces cerevisiae. The proximate and compositional analysis of BP and PP was obtained about 6.67% moisture, 5.75% ash, 82.75% volatile matter, and 5% fixed carbon and 1.1gram, 38.1%, 15.7% and 45.1% extractives, hemicellulose, lignin, and cellulose respectively from BP and 8.165% moisture, 5.5% ash, 81.25% volatile matter and 6% fixed carbon 2.08 gram, 42%, 8.6% and 47.32% extractives, hemicellulose, lignin, and cellulose respectively from PP. After Pretreat with KOH (5% w/v) optimize hydrolysis process parameters based on central composite design (CCD) to maximize fermentable sugars. The optimized hydrolysis conditions were 50:50 w/v% mixing of BP and PP, 1.75% H2SO4, and pH 5. The reducing sugar content was measured by DNS and results 11.737g/ml from fifty (50) grams of BP and PP. The maximum yield of bioethanol was 22.5% recorded after 72 hours. Fourier Transform Infrared Spectroscopy (FTIR) peaks associated with O-H, C-O, and C-H stretching and vibrations confirmed the presence of bioethanol in the product. The result confirms that the combination of BP and PP boosts bioethanol productivity than single peels.

VL  - 8
IS  - 2
ER  -

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Author Information

Asab Alemneh Tadesse

Ethiopian Institutes of Agricultural Research, National Agricultural Biotechnology Research Center, Holleta, Ethiopia

Contact Email

http://orcid.org/0009-0001-2117-4021
Temesgen Atnafu Yemata

Ethiopian Institutes of Agricultural Research, National Agricultural Biotechnology Research Center, Holleta, Ethiopia

http://orcid.org/0000-0002-5041-9665

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Plain Text BibTeX RIS

APA Style

Tadesse, A. A., Yemata, T. A. (2024). Production and Optimization of Bioethanol from Mixed Banana and Papaya Peels Using Saccharomyces Cerevisiae. American Journal of Chemical and Biochemical Engineering, 8(2), 45-57. https://doi.org/10.11648/j.ajcbe.20240802.12

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ACS Style

Tadesse, A. A.; Yemata, T. A. Production and Optimization of Bioethanol from Mixed Banana and Papaya Peels Using Saccharomyces Cerevisiae. Am. J. Chem. Biochem. Eng. 2024, 8(2), 45-57. doi: 10.11648/j.ajcbe.20240802.12

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AMA Style

Tadesse AA, Yemata TA. Production and Optimization of Bioethanol from Mixed Banana and Papaya Peels Using Saccharomyces Cerevisiae. Am J Chem Biochem Eng. 2024;8(2):45-57. doi: 10.11648/j.ajcbe.20240802.12

Copy | Download

@article{10.11648/j.ajcbe.20240802.12,
  author = {Asab Alemneh Tadesse and Temesgen Atnafu Yemata},
  title = {Production and Optimization of Bioethanol from Mixed Banana and Papaya Peels Using Saccharomyces Cerevisiae
},
  journal = {American Journal of Chemical and Biochemical Engineering},
  volume = {8},
  number = {2},
  pages = {45-57},
  doi = {10.11648/j.ajcbe.20240802.12},
  url = {https://doi.org/10.11648/j.ajcbe.20240802.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcbe.20240802.12},
  abstract = {Mixed fruit peels (Banana (BP) and Papaya (PP) bioethanol was produced using Saccharomyces cerevisiae. The proximate and compositional analysis of BP and PP was obtained about 6.67% moisture, 5.75% ash, 82.75% volatile matter, and 5% fixed carbon and 1.1gram, 38.1%, 15.7% and 45.1% extractives, hemicellulose, lignin, and cellulose respectively from BP and 8.165% moisture, 5.5% ash, 81.25% volatile matter and 6% fixed carbon 2.08 gram, 42%, 8.6% and 47.32% extractives, hemicellulose, lignin, and cellulose respectively from PP. After Pretreat with KOH (5% w/v) optimize hydrolysis process parameters based on central composite design (CCD) to maximize fermentable sugars. The optimized hydrolysis conditions were 50:50 w/v% mixing of BP and PP, 1.75% H2SO4, and pH 5. The reducing sugar content was measured by DNS and results 11.737g/ml from fifty (50) grams of BP and PP. The maximum yield of bioethanol was 22.5% recorded after 72 hours. Fourier Transform Infrared Spectroscopy (FTIR) peaks associated with O-H, C-O, and C-H stretching and vibrations confirmed the presence of bioethanol in the product. The result confirms that the combination of BP and PP boosts bioethanol productivity than single peels.
},
 year = {2024}
}

Copy | Download

TY  - JOUR
T1  - Production and Optimization of Bioethanol from Mixed Banana and Papaya Peels Using Saccharomyces Cerevisiae

AU  - Asab Alemneh Tadesse
AU  - Temesgen Atnafu Yemata
Y1  - 2024/09/29
PY  - 2024
N1  - https://doi.org/10.11648/j.ajcbe.20240802.12
DO  - 10.11648/j.ajcbe.20240802.12
T2  - American Journal of Chemical and Biochemical Engineering
JF  - American Journal of Chemical and Biochemical Engineering
JO  - American Journal of Chemical and Biochemical Engineering
SP  - 45
EP  - 57
PB  - Science Publishing Group
SN  - 2639-9989
UR  - https://doi.org/10.11648/j.ajcbe.20240802.12
AB  - Mixed fruit peels (Banana (BP) and Papaya (PP) bioethanol was produced using Saccharomyces cerevisiae. The proximate and compositional analysis of BP and PP was obtained about 6.67% moisture, 5.75% ash, 82.75% volatile matter, and 5% fixed carbon and 1.1gram, 38.1%, 15.7% and 45.1% extractives, hemicellulose, lignin, and cellulose respectively from BP and 8.165% moisture, 5.5% ash, 81.25% volatile matter and 6% fixed carbon 2.08 gram, 42%, 8.6% and 47.32% extractives, hemicellulose, lignin, and cellulose respectively from PP. After Pretreat with KOH (5% w/v) optimize hydrolysis process parameters based on central composite design (CCD) to maximize fermentable sugars. The optimized hydrolysis conditions were 50:50 w/v% mixing of BP and PP, 1.75% H2SO4, and pH 5. The reducing sugar content was measured by DNS and results 11.737g/ml from fifty (50) grams of BP and PP. The maximum yield of bioethanol was 22.5% recorded after 72 hours. Fourier Transform Infrared Spectroscopy (FTIR) peaks associated with O-H, C-O, and C-H stretching and vibrations confirmed the presence of bioethanol in the product. The result confirms that the combination of BP and PP boosts bioethanol productivity than single peels.

VL  - 8
IS  - 2
ER  -

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