In Vitro Endothelial Differenthelial Assessment on Polyglycerol Sebacate / Polycaprolactone /Gelatin Electospun Scaffold

Shima Kamalijoo; Kimia Ranjbar; Donya Lotfizadeh; Atousa Nazari

doi:doi:10.11648/j.ajcbe.20250901.13

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| Peer-Reviewed

In Vitro Endothelial Differenthelial Assessment on Polyglycerol Sebacate / Polycaprolactone /Gelatin Electospun Scaffold

Shima Kamalijoo

, Kimia Ranjbar^*

, Donya Lotfizadeh

, Atousa Nazari

Published in American Journal of Chemical and Biochemical Engineering (Volume 9, Issue 1)

Received: 27 December 2024 Accepted: 11 February 2025 Published: 29 May 2025

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Abstract

Due to the prevalence of cardiovascular diseases and their growing trend throughout the world, including Iran, efforts to treat these types of diseases have increased. There are lots of therapies for restoring cardiovascular function include organ transplants, reconstructive surgery, the use of mechanical or artificial devices, and the use of metabolic products. Although these methods are commonly used, they cause problems due to donor limitations, such as biocompatibility, infection, and tissue rejection by the patient. Meanwhile, vascular tissue engineering with the aim of building biocompatible and efficient vessels to replace lost vessels has created high hopes for the treatment of lesions. In this article, we used polyglycerol sebacate polymer (PGS) due to the properties such as high biocompatibility, good cell adhesion, controllable degradation rate and desirable mechanical properties, and combined it with polycaprolactone polymer (PCL) and gelatin to fabricate 3D scaffolds by electrospinning method. We also added vascular endothelial growth factor and then analysed the endothelial differentiation of mesenchymal stem cells. The expression of CD31 and VEGF-R2 genes has been measured by qPCR method, which revealed reasonable results.

Published in	American Journal of Chemical and Biochemical Engineering (Volume 9, Issue 1)
DOI	10.11648/j.ajcbe.20250901.13
Page(s)	38-47
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2025. Published by Science Publishing Group

Keywords

PGS, PCL, Gelatin, Stem Cells, Differentiation, Endothelial Differentiation, Electrospinning, In Vitro

References

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APA Style

Kamalijoo, S., Ranjbar, K., Lotfizadeh, D., Nazari, A. (2025). In Vitro Endothelial Differenthelial Assessment on Polyglycerol Sebacate / Polycaprolactone /Gelatin Electospun Scaffold. American Journal of Chemical and Biochemical Engineering, 9(1), 38-47. https://doi.org/10.11648/j.ajcbe.20250901.13

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ACS Style

Kamalijoo, S.; Ranjbar, K.; Lotfizadeh, D.; Nazari, A. In Vitro Endothelial Differenthelial Assessment on Polyglycerol Sebacate / Polycaprolactone /Gelatin Electospun Scaffold. Am. J. Chem. Biochem. Eng. 2025, 9(1), 38-47. doi: 10.11648/j.ajcbe.20250901.13

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AMA Style

Kamalijoo S, Ranjbar K, Lotfizadeh D, Nazari A. In Vitro Endothelial Differenthelial Assessment on Polyglycerol Sebacate / Polycaprolactone /Gelatin Electospun Scaffold. Am J Chem Biochem Eng. 2025;9(1):38-47. doi: 10.11648/j.ajcbe.20250901.13

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@article{10.11648/j.ajcbe.20250901.13,
  author = {Shima Kamalijoo and Kimia Ranjbar and Donya Lotfizadeh and Atousa Nazari},
  title = {In Vitro Endothelial Differenthelial Assessment on Polyglycerol Sebacate / Polycaprolactone /Gelatin Electospun Scaffold
},
  journal = {American Journal of Chemical and Biochemical Engineering},
  volume = {9},
  number = {1},
  pages = {38-47},
  doi = {10.11648/j.ajcbe.20250901.13},
  url = {https://doi.org/10.11648/j.ajcbe.20250901.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcbe.20250901.13},
  abstract = {Due to the prevalence of cardiovascular diseases and their growing trend throughout the world, including Iran, efforts to treat these types of diseases have increased. There are lots of therapies for restoring cardiovascular function include organ transplants, reconstructive surgery, the use of mechanical or artificial devices, and the use of metabolic products. Although these methods are commonly used, they cause problems due to donor limitations, such as biocompatibility, infection, and tissue rejection by the patient. Meanwhile, vascular tissue engineering with the aim of building biocompatible and efficient vessels to replace lost vessels has created high hopes for the treatment of lesions. In this article, we used polyglycerol sebacate polymer (PGS) due to the properties such as high biocompatibility, good cell adhesion, controllable degradation rate and desirable mechanical properties, and combined it with polycaprolactone polymer (PCL) and gelatin to fabricate 3D scaffolds by electrospinning method. We also added vascular endothelial growth factor and then analysed the endothelial differentiation of mesenchymal stem cells. The expression of CD31 and VEGF-R2 genes has been measured by qPCR method, which revealed reasonable results.
},
 year = {2025}
}

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TY  - JOUR
T1  - In Vitro Endothelial Differenthelial Assessment on Polyglycerol Sebacate / Polycaprolactone /Gelatin Electospun Scaffold

AU  - Shima Kamalijoo
AU  - Kimia Ranjbar
AU  - Donya Lotfizadeh
AU  - Atousa Nazari
Y1  - 2025/05/29
PY  - 2025
N1  - https://doi.org/10.11648/j.ajcbe.20250901.13
DO  - 10.11648/j.ajcbe.20250901.13
T2  - American Journal of Chemical and Biochemical Engineering
JF  - American Journal of Chemical and Biochemical Engineering
JO  - American Journal of Chemical and Biochemical Engineering
SP  - 38
EP  - 47
PB  - Science Publishing Group
SN  - 2639-9989
UR  - https://doi.org/10.11648/j.ajcbe.20250901.13
AB  - Due to the prevalence of cardiovascular diseases and their growing trend throughout the world, including Iran, efforts to treat these types of diseases have increased. There are lots of therapies for restoring cardiovascular function include organ transplants, reconstructive surgery, the use of mechanical or artificial devices, and the use of metabolic products. Although these methods are commonly used, they cause problems due to donor limitations, such as biocompatibility, infection, and tissue rejection by the patient. Meanwhile, vascular tissue engineering with the aim of building biocompatible and efficient vessels to replace lost vessels has created high hopes for the treatment of lesions. In this article, we used polyglycerol sebacate polymer (PGS) due to the properties such as high biocompatibility, good cell adhesion, controllable degradation rate and desirable mechanical properties, and combined it with polycaprolactone polymer (PCL) and gelatin to fabricate 3D scaffolds by electrospinning method. We also added vascular endothelial growth factor and then analysed the endothelial differentiation of mesenchymal stem cells. The expression of CD31 and VEGF-R2 genes has been measured by qPCR method, which revealed reasonable results.

VL  - 9
IS  - 1
ER  -

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Author Information

Shima Kamalijoo

Department of Materials Engineering, Isfahan University, Isfahan, Iran

http://orcid.org/0000-0002-8408-260X
Kimia Ranjbar

Shiraz Pasargad Higher Education Institute, Shiraz, Iran

Contact Email

http://orcid.org/0000-0001-6548-3304
Donya Lotfizadeh

Islamic Azad University, Najafabad University, Isfahan, Iran

http://orcid.org/0000-0003-4181-7917
Atousa Nazari

null

http://orcid.org/0000-0001-5354-5424

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Plain Text BibTeX RIS

APA Style

Kamalijoo, S., Ranjbar, K., Lotfizadeh, D., Nazari, A. (2025). In Vitro Endothelial Differenthelial Assessment on Polyglycerol Sebacate / Polycaprolactone /Gelatin Electospun Scaffold. American Journal of Chemical and Biochemical Engineering, 9(1), 38-47. https://doi.org/10.11648/j.ajcbe.20250901.13

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ACS Style

Kamalijoo, S.; Ranjbar, K.; Lotfizadeh, D.; Nazari, A. In Vitro Endothelial Differenthelial Assessment on Polyglycerol Sebacate / Polycaprolactone /Gelatin Electospun Scaffold. Am. J. Chem. Biochem. Eng. 2025, 9(1), 38-47. doi: 10.11648/j.ajcbe.20250901.13

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AMA Style

Kamalijoo S, Ranjbar K, Lotfizadeh D, Nazari A. In Vitro Endothelial Differenthelial Assessment on Polyglycerol Sebacate / Polycaprolactone /Gelatin Electospun Scaffold. Am J Chem Biochem Eng. 2025;9(1):38-47. doi: 10.11648/j.ajcbe.20250901.13

Copy | Download

@article{10.11648/j.ajcbe.20250901.13,
  author = {Shima Kamalijoo and Kimia Ranjbar and Donya Lotfizadeh and Atousa Nazari},
  title = {In Vitro Endothelial Differenthelial Assessment on Polyglycerol Sebacate / Polycaprolactone /Gelatin Electospun Scaffold
},
  journal = {American Journal of Chemical and Biochemical Engineering},
  volume = {9},
  number = {1},
  pages = {38-47},
  doi = {10.11648/j.ajcbe.20250901.13},
  url = {https://doi.org/10.11648/j.ajcbe.20250901.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcbe.20250901.13},
  abstract = {Due to the prevalence of cardiovascular diseases and their growing trend throughout the world, including Iran, efforts to treat these types of diseases have increased. There are lots of therapies for restoring cardiovascular function include organ transplants, reconstructive surgery, the use of mechanical or artificial devices, and the use of metabolic products. Although these methods are commonly used, they cause problems due to donor limitations, such as biocompatibility, infection, and tissue rejection by the patient. Meanwhile, vascular tissue engineering with the aim of building biocompatible and efficient vessels to replace lost vessels has created high hopes for the treatment of lesions. In this article, we used polyglycerol sebacate polymer (PGS) due to the properties such as high biocompatibility, good cell adhesion, controllable degradation rate and desirable mechanical properties, and combined it with polycaprolactone polymer (PCL) and gelatin to fabricate 3D scaffolds by electrospinning method. We also added vascular endothelial growth factor and then analysed the endothelial differentiation of mesenchymal stem cells. The expression of CD31 and VEGF-R2 genes has been measured by qPCR method, which revealed reasonable results.
},
 year = {2025}
}

Copy | Download

TY  - JOUR
T1  - In Vitro Endothelial Differenthelial Assessment on Polyglycerol Sebacate / Polycaprolactone /Gelatin Electospun Scaffold

AU  - Shima Kamalijoo
AU  - Kimia Ranjbar
AU  - Donya Lotfizadeh
AU  - Atousa Nazari
Y1  - 2025/05/29
PY  - 2025
N1  - https://doi.org/10.11648/j.ajcbe.20250901.13
DO  - 10.11648/j.ajcbe.20250901.13
T2  - American Journal of Chemical and Biochemical Engineering
JF  - American Journal of Chemical and Biochemical Engineering
JO  - American Journal of Chemical and Biochemical Engineering
SP  - 38
EP  - 47
PB  - Science Publishing Group
SN  - 2639-9989
UR  - https://doi.org/10.11648/j.ajcbe.20250901.13
AB  - Due to the prevalence of cardiovascular diseases and their growing trend throughout the world, including Iran, efforts to treat these types of diseases have increased. There are lots of therapies for restoring cardiovascular function include organ transplants, reconstructive surgery, the use of mechanical or artificial devices, and the use of metabolic products. Although these methods are commonly used, they cause problems due to donor limitations, such as biocompatibility, infection, and tissue rejection by the patient. Meanwhile, vascular tissue engineering with the aim of building biocompatible and efficient vessels to replace lost vessels has created high hopes for the treatment of lesions. In this article, we used polyglycerol sebacate polymer (PGS) due to the properties such as high biocompatibility, good cell adhesion, controllable degradation rate and desirable mechanical properties, and combined it with polycaprolactone polymer (PCL) and gelatin to fabricate 3D scaffolds by electrospinning method. We also added vascular endothelial growth factor and then analysed the endothelial differentiation of mesenchymal stem cells. The expression of CD31 and VEGF-R2 genes has been measured by qPCR method, which revealed reasonable results.

VL  - 9
IS  - 1
ER  -

Copy | Download